Ptriex-mcherry-lov2
WebLOV2-Controlled Photoactivation of Protein Trans -Splicing Anam Qudrat , Abdullah Mosabbir , and Kevin ruong T Abstract Protein trans -splicing is a posttranslational modiÞ cation that joins two protein fragments together via a ... pTriEx-mCherry-PA-Rac1 (Addgene Plasmid #22027) as a source for the LOV2 gene. 3. Restriction enzymes and ... WebAddgene inc ptriex mcherry zdk1 Ptriex Mcherry Zdk1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Ptriex-mcherry-lov2
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WebJan 29, 2024 · Because Zdk1 binding requires the LOV2 C terminus, we further inserted mCherry between EB1N and LOV2 to verify that the π-EB1 N-terminal half remained on … WebWe have found that 10:1 ratio of pTriEX-N-TOM20-LOV2 and pTriEx-mVenus-Zdk1-VAV2 DHPHC1 [1, 17] produces good optogenetic activation in living cells. ... This problem can be circumvented by switching the tag to mtagBFP2 and exciting it at 395 nm, or mCherry (585 nm) if longer wavelengths can be used. Trans-illumination light train for DIC may ...
WebJan 1, 2024 · pTriEX-NTOM20-LOV2 (Addgene #81009) LATT N-terminus: pTriEX-mCherry-Zdk1 (Addgene #81057) 2.2. Cell culture. ... Transient transfection with untagged EB1N-LOV2 and mCherry-Zdk1-EB1C is a simple and useful way to characterize the photoactivation setup. However, for making cell lines in which endogenous EB1 is … WebJan 1, 2024 · Europe PMC is an archive of life sciences journal literature.
WebJan 27, 2024 · For application of the LOVTRAP or CRY2-CIB1 system, Nanoluc was ligated into the pTriEx-mCherry-LOV2 (Addgene: 81041) plasmid digested by Acc65I and NheI … WebFeb 8, 2016 · Abstract. Active nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of ...
WebMay 18, 2024 · The Light-oxygen-voltage-sensing domain 2 (LOV2) sequence was amplified from pTriEx-NTOM20-LOV plasmid, Zdark 1 (Zdk1) from pTriEX-mCherry-Zdk1, and …
WebpTriEx-mCherry-LOV2. PVT13411 2ug . pTriEx-mCherry-LOV2 Information. Bacterial Resistance(s): Amp. Screening: Growth Strain(s): DH5 alpha Growth Condition: LB/37 … iec 60502 cable specificationWeb© 2024-2024 MolecularCloud All rights reserved. iec 60529 standard pdf free downloadWebPlasmid pTriEx-mCherry-LOV2 V416L from Dr. Klaus Hahn's lab contains the insert LOV V416L and is published in Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. This … is sharex open sourceWebJan 27, 2024 · For application of the LOVTRAP or CRY2-CIB1 system, Nanoluc was ligated into the pTriEx-mCherry-LOV2 (Addgene: 81041) plasmid digested by Acc65I and NheI … iec 60571 pdf free downloadWebPlasmid pTriEx-mCherry-LOV2 I427T from Dr. Klaus Hahn's lab contains the insert LOV I427T and is published in Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. This … iec 60534-4 class ivWebJul 18, 2016 · Mammalian codon-optimized Amg2 (BioBasic) with a C-terminal mCherry or N-terminal tagRFP was subcloned into pTRIEX (a gift from K. Hahn, Addgene plasmid 81041) 7 Imaging was carried out using a ... is shareworks a transfer agentWebDec 8, 2015 · Fusion with the lightswitch LOV2 domain confers photosensitivity to the ORAI1-activating STIM1-CT fragments. ... Schematic of the MBP- or mCherry (mCh)-tagged LOVSoc constructs used in the pulldown or CoIP assays. ... (macrophage) and embryo (MEF). Cultured cells were transiently transfected by 100 ng pTriEx-mCh-LOVSoc or … is shareware junction safe