Peasy e1
WebpEASY-Blunt E1 (linearized) Sequence and Map Prokaryotic expression vector for cloning blunt-ended PCR products and adding an N-terminal 6xHis tag. Prokaryotic expression … WebMar 18, 2024 · The members of the Old Yellow Enzyme (OYE) family are capable of catalyzing the asymmetric reduction of ( E / Z )-citral to ( R )-citronellal—a key intermediate in the synthesis of L-menthol. The applications of OYE-mediated biotransformation are usually hampered by its insufficient enantioselectivity and low activity.
Peasy e1
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WebSep 2, 2024 · The recombinant TaTCP-1 protein was ectopically expressed in E.coli following transformation with the pEASY-E1-TaTCP-1 and the control plasmid pEASY. Crude … WebJul 26, 2024 · The PCR products were cloned into pEASY-E1 expression vectors (Transgen, Beijing, China). BL21 (DE3) competent cells were transformed with the pEASY-E1 …
WebOct 18, 2024 · The expression vector pEASY-E1 (TransGen biotech, China) carrying a C-terminal His6-tag was used to clone and express catA. Cloning and sequencing of catA from R. ruber OA1: Rhodococcus ruber strain OA1 was cultured in LB broth at 37°C for 2 days and genomic DNA was extracted using the Bacterial gDNA Kit (BIOMIGA, China) for use as a … WebpEASY-Blunt E1 (linearized) Prokaryotic expression vector for cloning blunt-ended PCR products and adding an N-terminal 6xHis tag. Sequence Author: TransBionovo (TransGen …
WebEasy Peasy is a custom distro based on Ubuntu 8.10 build for EeePC series. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How …
WebAug 15, 2012 · The recombinant plasmid (pEASY-E1-CfC1qDC) was transformed into E. coli BL21 (DE3)-Transetta (Transgen). After IPTG induction for 4 h, the whole cell lysate was analyzed by SDS-PAGE, and a distinct band was revealed with a molecular weight of 17 kDa ( Figure 3 ), which was in accordance with the previous predicted molecular weight 17.2 …
WebJul 1, 2012 · Amplified PCR products were digested with the respective restriction enzymes and cloned in frame with pEasy-E1 vector (TransGen, Beijing, China), resulting in pEasy-E1-CsTS. After confirming the cloned fragments by DNA sequencing, pEasy-E1-CsTS was transformed into the Rosseta good (DE3) cells and the recombinant CsTS protein … christopher henryWebRecombinant plasmid pEASY-E1-TNFα was transformed into E. coli BL21 (DE3) to obtain recombinant Ss_TNFα protein. The transformant BL21-TNFα and the control BL21 with empty pEASY-E1 were cultured in LB-kanamycin at 37 °C with shaking at 200 rpm. When the culture medium reached an OD600 of 0.4 – 0.6, IPTG was added to the medium at a final ... christopher henn surveyorWebJun 5, 2013 · After confirming the cloned fragments by DNA sequencing, pEasy-E1-CsTS was transformed into Rosetta Gami (DE3) cells and the recombinant CsTS protein was … getting rid of lower back fathttp://www.zgenebio.com.tw/uploads/2/6/9/2/26924153/peasy_-e1_kit.pdf christopher henry gayleWeberichiacoli and pEASY-E1 (T vector) were purchased from Transgen(Beijing,China).PronasewasfromM&CGeneTech-nology (Beijing, China). Cell Lines—Human lung adenocarcinoma cell line A549, human hepatocellular carcinoma cell line HepG2, human mammary epithelial cell line MCF10A, and human breast car-cinoma … christopher henning sycamoreWebApr 13, 2024 · 将pEASY®-Blunt E1-Brachyury导入Trans 10大肠杆菌感受态细胞中，用SOB无菌液体培养基(含100 μg/mL氨苄青霉素)于37 ℃、200 r/min条件下扩增培养1 h。取菌液涂布于LB固体培养基中，置37 ℃生化培养箱中倒置培养14 h后，挑取单克隆菌落加入SOB无菌液体培养基(含100 μg/mL氨苄 ... christopher henn-collinsWebOct 29, 2024 · The 2952-bp styS gene was amplified by PCR from the P. putida B3 genome using primers F/R-pEASY-styS ( Table 2 ), and the recombined plasmid pEASY-E1-styS was then constructed. Subsequently, the pEASY-E1-styS vector was introduced into E. coli BL21 (DE3), and the StyS protein was obtained by adopting the same method used for the StyR … christopher hennessy realtor